Direct demonstration and quantification of long-range DNA looping by the lambda bacteriophage repressor.
about
Predicting gene-regulation functions: lessons from temperate bacteriophagesTranscription-factor-mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cellsSpatial and topological organization of DNA chains induced by gene co-localizationEffect of promoter architecture on the cell-to-cell variability in gene expressionSingle-molecule approaches to probe the structure, kinetics, and thermodynamics of nucleoprotein complexes that regulate transcription.High-throughput single-molecule analysis of DNA-protein interactions by tethered particle motion.Purification of bacteriophage lambda repressorMADS domain transcription factors mediate short-range DNA looping that is essential for target gene expression in Arabidopsis.Aspects of protein-DNA interactions: a review of quantitative thermodynamic theory for modelling synthetic circuits utilising LacI and CI repressors, IPTG and the reporter gene lacZ.DNA supercoiling: a regulatory signal for the λ repressorHU protein induces incoherent DNA persistence length.Multilevel autoregulation of λ repressor protein CI by DNA looping in vitroE. coli Gyrase Fails to Negatively Supercoil Diaminopurine-Substituted DNA.Nonconsensus Protein Binding to Repetitive DNA Sequence Elements Significantly Affects Eukaryotic GenomesA genetic network that balances two outcomes utilizes asymmetric recognition of operator sites.Enhancer-like long-range transcriptional activation by λ CI-mediated DNA loopingOptical Methods to Study Protein-DNA Interactions in Vitro and in Living Cells at the Single-Molecule Level.Physiological levels of salt and polyamines favor writhe and limit twist in DNAAFM studies of lambda repressor oligomers securing DNA loopsProbing DNA topology using tethered particle motion.Single molecule analysis of DNA wrapping and looping by a circular 14mer wheel of the bacteriophage 186 CI repressorDNA supercoiling enhances cooperativity and efficiency of an epigenetic switch.Positive and negative design for nonconsensus protein-DNA binding affinity in the vicinity of functional binding sites.Quantitation of the DNA tethering effect in long-range DNA looping in vivo and in vitro using the Lac and λ repressors.Enhanced tethered-particle motion analysis reveals viscous effectsTethered particle motion reveals that LacI·DNA loops coexist with a competitor-resistant but apparently unlooped conformation.Tethered particle analysis of supercoiled circular DNA using peptide nucleic acid handles.Cooperativity leads to temporally-correlated fluctuations in the bacteriophage lambda genetic switch.Stretching DNA to quantify nonspecific protein binding.Determination of the number of proteins bound non-specifically to DNA.Quantitative analysis of DNA-looping kinetics from tethered particle motion experiments.The effect of nonspecific binding of lambda repressor on DNA looping dynamics.Effect of supercoiling on the λ switch.Bacteriophage lambda repressor mediates the formation of a complex enhancer-like structure.Nonspecific protein-DNA binding is widespread in the yeast genome.Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Interactions with Transcription Factors.Specifically bound lambda repressor dimers promote adjacent non-specific binding.
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P2860
Direct demonstration and quantification of long-range DNA looping by the lambda bacteriophage repressor.
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article científic
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article scientifique
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articolo scientifico
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artigo científico
@pt
bilimsel makale
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scientific article published on 10 March 2009
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Direct demonstration and quant ...... ambda bacteriophage repressor.
@en
Direct demonstration and quant ...... ambda bacteriophage repressor.
@nl
type
label
Direct demonstration and quant ...... ambda bacteriophage repressor.
@en
Direct demonstration and quant ...... ambda bacteriophage repressor.
@nl
prefLabel
Direct demonstration and quant ...... ambda bacteriophage repressor.
@en
Direct demonstration and quant ...... ambda bacteriophage repressor.
@nl
P2093
P2860
P356
P1476
Direct demonstration and quant ...... lambda bacteriophage repressor
@en
P2093
Chiara Zurla
Dale E A Lewis
David Dunlap
Laura Finzi
Sankar Adhya
P2860
P304
P356
10.1093/NAR/GKP134
P407
P50
P577
2009-03-10T00:00:00Z