about
Nature's recipe for splitting inteinsStructure of cyclized green fluorescent proteinStructural Basis for Complement Evasion by Lyme Disease Pathogen Borrelia burgdorferiNMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous InteinIntermolecular domain swapping induces intein-mediated protein alternative splicingStructure-based engineering and comparison of novel split inteins for protein ligationHidden photoinduced reactivity of the blue fluorescent protein mKalama1Salt-inducible protein splicing in cis and trans by inteins from extremely halophilic archaea as a novel protein-engineering toolNMR structures of salt-refolded forms of the 434-repressor DNA-binding domain in 6 M urea.Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA inteinSolution conformation of an antibacterial peptide, sarcotoxin IA, as determined by 1H-NMR.Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.Protein ligation: applications in NMR studies of proteins.Protein trans-splicing and its use in structural biology: opportunities and limitations.Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.Conformational dynamics as a key factor of signaling mediated by the receiver domain of sensor histidine kinase from Arabidopsis thaliana.Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.NMR resonance assignment of DnaE intein from Nostoc punctiforme.Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR.Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA.Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins.Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR.Hideo Iwai. Interview by Daniela Ruffell.Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase.Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR.Tandem SUMO fusion vectors for improving soluble protein expression and purification.Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli.Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.Use of biosynthetic fractional 13C-labeling for backbone NMR assignment of proteins.Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD.Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligationNMR solution structure of the monomeric form of the bacteriophage λ capsid stabilizing protein gpDCircular β-lactamase: stability enhancement by cyclizing the backboneNMR structure of the C-terminal domain of TonB protein fromRotational Dynamics of Proteins from Spin Relaxation Times and Molecular Dynamics SimulationsSegmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-SplicingHighly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiformeThe 2D NMR experiments H(C)CO2 and HCCO2 for assignment and pH titration of carboxylate groups in uniformly 15N/13C-labeled proteins
P50
Q26830327-A5A1F6B2-F1DC-4127-BF5F-76338C1460C7Q27639539-84E4800D-77C1-4127-BE57-6ECE77B201B1Q27678051-6017F4F5-747F-422A-A1CE-0E1493231731Q27678916-C3B0CC50-6F51-4C86-9F24-DE850F4B6F77Q27685412-4CB9F43F-0559-4A16-A97B-5F69B9474483Q27689357-ADF0C893-AEAB-47FB-A29D-53C331E3D932Q27700035-7EAE9EF5-E1AE-4562-BE30-CE72EDDF78E4Q27728247-9FE83DAC-B221-4130-8C08-A410BA8565FAQ30974562-7A15187E-31EE-4278-9892-F47B938DC2B5Q33890282-C8C51B3D-CFE8-4FE0-AE91-0B3DC022F68AQ34343605-132CF45B-D964-4D0C-B08A-3DA058DCCD83Q36117328-78EA3ECB-E33B-4051-AB44-CE513E6CB86DQ37024681-F558276D-2C00-49B0-9797-ECC159C9716CQ37785771-1A5BE734-EAB3-4971-8744-E0AFF6A30342Q38320323-FB3066E9-9594-48F7-BC2E-2C1DB2378838Q38599177-FC37BDB5-57DD-4327-A552-43041229DC81Q43560102-9746B9CF-8F47-4384-9A51-0CBF0CD33D6AQ45154185-252E65AD-3E61-4513-9B85-7BB87C8E85A0Q47139950-CD6C7F7C-450A-4053-A282-F85547DC8AF2Q47279190-15CBAF42-41DE-4407-B1EA-EDFD107EB8F0Q47620717-7AF49EC6-6FA6-4B33-B844-43098FCF46F7Q48240503-3B4D6F16-7B2D-444C-AA1C-E8710FF23F4FQ48308155-CBE264B4-0EE6-4689-8572-BE58449CD498Q50934500-BC9B8F08-2B1B-4162-B312-E5E3B177DEFFQ52641705-FA5DE174-5155-43EF-809E-2B740B569724Q54261919-D88E7728-103E-406A-A780-530AB741B621Q54350774-7FC654FD-A0FE-4A2B-A98F-583ED026EB18Q54389645-680D40E9-CB21-45D2-9054-4B1824D6EA5AQ54448553-1CBBB502-0C89-4651-BC05-9A53338B83C5Q54485829-FAC58E5C-1409-4E8F-989B-D8E08B10AEECQ54512014-C530C509-083D-450D-AE06-6D41A9D98966Q54733646-2594479E-C244-4E79-8B8D-BE96F46BE922Q57839819-9CE11944-81F4-431F-803F-8CFB06E52DD6Q57839970-8507BB76-63CB-4EF5-BF4A-6F57F811DA38Q57840029-1AB60113-2BB6-47BC-8995-E542C3EDCB3CQ58700950-576D4F69-3A9D-4C23-869F-4C308B0A51D0Q58761623-87172D26-7531-413A-9DB9-602A7D4504B5Q58761727-1083C596-C83F-4B13-9F8B-D56BEBEC4424Q58761739-B07F332B-136A-45CC-938B-2D7212FC984FQ74020850-BFA5144E-3C44-4353-A9F9-E5804F260E4C
P50
description
hulumtues
@sq
onderzoeker
@nl
researcher
@en
հետազոտող
@hy
name
Hideo Iwai
@ast
Hideo Iwai
@en
Hideo Iwai
@es
Hideo Iwai
@nl
Hideo Iwai
@sl
type
label
Hideo Iwai
@ast
Hideo Iwai
@en
Hideo Iwai
@es
Hideo Iwai
@nl
Hideo Iwai
@sl
prefLabel
Hideo Iwai
@ast
Hideo Iwai
@en
Hideo Iwai
@es
Hideo Iwai
@nl
Hideo Iwai
@sl
P1053
A-6416-2009
P106
P21
P31
P3829
P496
0000-0001-7376-5264