Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.Plating Bacteriophage M13.Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol.Isolation of High-Molecular-Weight DNA from Monolayer Cultures of Mammalian Cells Using Proteinase K and Phenol.Isolation of High-Molecular-Weight DNA Using Organic Solvents.Isolation of High-Molecular-Weight DNA from Mammalian Tissues Using Proteinase K and Phenol.A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues.Growing Bacteriophage M13 in Liquid Culture.Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol.Concentrating Nucleic Acids by Extraction with Butanol.Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps.Buffers.Caring for Escherichia coli.One-Tube Isolation of DNA from Mouse Tails.Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA.Preparation of Genomic DNA from Mouse Tails and Other Small Samples.Precipitation of DNA with Isopropanol.Isolating DNA from Gram-Negative Bacteria.Precipitation of DNA with Ethanol.Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps.Two ATM variants and breast cancer risk.Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental DataQuantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) ExperimentsOptimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) AssaysEcdysone as regulator of inducible gene expression in Mammalian cellsRecovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose MembranesMarkers for Pulsed-field Gel ElectrophoresisIsolation of High-molecular-weight DNA from Mammalian Cells Using FormamidePreparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from YeastPreparation of Double-stranded (Replicative Form) Bacteriophage M13 DNALarge-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNAScreening an Unamplified Cosmid Library by Hybridization: Plating the Library onto FiltersWorking with yeast artificial chromosomesGrowth of S. cerevisiae and Preparation of DNASmall-scale Preparations of Yeast DNAConstruction of cDNA libraries * stage 4: attachment of linkers or adaptorsRemoval of Cross-reactive Antibodies from Antiserum: Affinity ChromatographyRapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method
P50
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P50
description
British molecular biologist
@en
Brits moleculair bioloog
@nl
britisch-australischer Molekularbiologe und Krebsforscher
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name
Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joe Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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Joseph Sambrook
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P5034
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P1153
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