High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.
about
GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migrationHigh production of fatty alcohols in Escherichia coli with fatty acid starvation.Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation.Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis.A genomic toolkit to investigate kinesin and myosin motor function in cells.Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker.Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli.Liquid-based iterative recombineering method tolerant to counter-selection escapes.Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coliDifferential requirements of singleplex and multiplex recombineering of large DNA constructs.A gene network engineering platform for lactic acid bacteria.PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli.GTSE1 tunes microtubule stability for chromosome alignment and segregation by inhibiting the microtubule depolymerase MCAK.Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis.Rational optimization of tolC as a powerful dual selectable marker for genome engineering.Recombineering to homogeneity: extension of multiplex recombineering to large-scale genome editing.Improved seamless mutagenesis by recombineering using ccdB for counterselectionRational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo.TACC3 protein regulates microtubule nucleation by affecting γ-tubulin ring complexes.Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and ActivatedInterrogation of Streptomyces avermitilis for efficient production of avermectins.A dual role of the extracellular domain of Drosophila Crumbs for morphogenesis of the embryonic neuroectoderm.
P2860
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P2860
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.
description
2011 nî lūn-bûn
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2011年の論文
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2011年学术文章
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2011年学术文章
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2011年学术文章
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2011年学术文章
@zh-my
2011年学术文章
@zh-sg
2011年學術文章
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2011年學術文章
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2011年學術文章
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name
High-efficiency counterselecti ...... terial artificial chromosomes.
@en
High-efficiency counterselecti ...... terial artificial chromosomes.
@nl
type
label
High-efficiency counterselecti ...... terial artificial chromosomes.
@en
High-efficiency counterselecti ...... terial artificial chromosomes.
@nl
prefLabel
High-efficiency counterselecti ...... terial artificial chromosomes.
@en
High-efficiency counterselecti ...... terial artificial chromosomes.
@nl
P2093
P2860
P356
P1433
P1476
High-efficiency counterselecti ...... terial artificial chromosomes.
@en
P2093
A Francis Stewart
Alexander W Bird
Axel Erler
Marcello Maresca
Youming Zhang
P2860
P2888
P304
P356
10.1038/NMETH.1803
P577
2011-12-04T00:00:00Z