The 1964 VFL Night Premiership Cup was the Victorian Football League end of season cup competition played in August and September of the 1964 VFL Premiership Season. Run as a knock-out tournament, it was contested by the eight VFL teams that failed to make the 1964 VFL finals series. It was the ninth VFL Night Series competition. Games were played at the Lake Oval, Albert Park, then the home ground of South Melbourne, as it was the only ground equipped to host night games. Footscray won its second consecutive night series cup defeating St Kilda in the final by 5 points.