The 2010 Segunda División B play-offs (Playoffs de Ascenso or Promoción de Ascenso) were the final playoffs for promotion from 2009–10 Segunda División B to the 2010–11 Segunda División. The four first placed teams in each of the four Segunda División B groups played the Playoffs de Ascenso and the four last placed teams in Segunda División were relegated to Segunda División B. It also decided the two teams which placed 16th to be relegated to the 2010–11 Tercera División.