The 28th Virginia Volunteer Infantry Regiment was an infantry regiment raised in Virginia for service in the Confederate States Army during the American Civil War. It fought mostly with the Army of Northern Virginia. The 28th Virginia completed its organization at Lynchburg, Virginia, in June, 1861. Its members were raised in the counties of Botetourt, Craig, Bedford, Campbell, and Roanoke. The field officers were Colonels Robert C. Allen, Robert T. Preston, and William Watts; Lieutenant Colonels Samuel B. Paul and William L. Wingfield; and Majors Michael P. Spesard and Nathaniel C. Wilson.