Diplorickettsia massiliensis species is an obligate intracellular, gram negative bacterium isolated from Ixodes ricinus ticks collected in Slovak republic forest geographically from southeastern part of Rovinka in 2006. They belong to the gammaproteobacteria class and are non endospore forming, small rods usually grouped in pairs. The bacteria are non-motile, and 16S rRNA, rpoB, parC and ftsY gene sequencing indicate that this bacterium is clearly different from all other recognized species. An initial phylogenetic analysis based on 16S rRNA, clustered D. massiliensis with Rickettsiella grylli. Because of its low 16S rDNA similarity (94%) with R. grylli, it was classified as a new genus Diplorickettsia into the family Coxiellaceae and the order Legionellales. D. massiliensis strain 20B was