The women's 4×100 metre medley relay event at the 2000 Summer Olympics took place on 22–23 September at the Sydney International Aquatic Centre in Sydney, Australia. The U.S. women's team established a new world record to defend their Olympic title in the event for the third consecutive streak. Leading the race from the start, Barbara Bedford (1:01.39), Megan Quann (1:06.29), Jenny Thompson (57.25), and Dara Torres (53.37) put together in a sterling time of 3:58.30 to clear the four-minute barrier and to smash China's six-year-old world record by 3.37 seconds. Capturing another relay title for the Americans, Thompson also picked up her eighth gold medal and tenth career as the nation's most successful female athlete in Olympic history.