The Trillium Railway (reporting mark TRRY) is a Canadian short-line railroad operating in the province of Ontario. Much of its right-of-way in the Niagara area was formerly part of the "Welland Canal Railway" (also known as the Welland Railway) that closely followed the route of the second Welland Canal. Trillium Railway began operations in 1997 with only eleven kilometres of railway, all of which were located in Port Colborne.