The 1859 Town of Dunedin by-election was a New Zealand by-election held in the single-member electorate of Town of Dunedin during the 2nd New Zealand Parliament on 14 January 1859, after the resignation of James Macandrew. The election was won by Macandrew, who had resigned from his seat on 2 November 1858 as a result of not attending a parliamentary session earlier in his term.