The 1964 Danish 2nd Division (Danish: Danmarksturneringens 2. division 1964) was the twenty-ninth season of the Danish second-tier association football division since the establishment of Danmarksturneringen's nation-wide league structure in 1927. Governed by the Danish FA, the season was launched on 29 March 1964, with the match between Ikast FS and Vanløse IF, and the last round of matches concluded in November 1964. Aalborg BK and Køge BK entered as relegated teams from last season's top-flight, while Hvidovre IF and Næstved IF entered as promoted teams from the . Fixtures for the 1964 season were announced in February 1964.