The 1965 Greenville 200 was a NASCAR Grand National Series event that was held on April 17, 1965, at Greenville-Pickens Speedway in Greenville, South Carolina. The transition to purpose-built racecars began in the early 1960s and occurred gradually over that decade. Changes made to the sport by the late 1960s brought an end to the "strictly stock" vehicles of the 1950s.