2000–01 KNVB Cup

The 83rd edition of the KNVB Cup (at the time called Amstel Cup) started on August 8, 2000. The final was played on May 24, 2001: FC Twente beat PSV on penalties (4–3), after no goals were made during official and extra time. FC Twente won the cup for the second time. A total of 86 clubs participated.

2000–01 KNVB Cup

The 83rd edition of the KNVB Cup (at the time called Amstel Cup) started on August 8, 2000. The final was played on May 24, 2001: FC Twente beat PSV on penalties (4–3), after no goals were made during official and extra time. FC Twente won the cup for the second time. A total of 86 clubs participated.