2000–01 Maltese First Division

The Maltese First Division 2000–01 (known as Rothmans First Division 2000–01 for sponsorship reasons) started on 9 September 2000 and finished on 29 April 2001. Gozo and Zurrieq were the relegated teams from the Maltese Premier League 1999-00. Marsaxlokk and Qormi were the promoted teams from the Maltese Second Division. Marsa finished as champions, by just one point, and were promoted to the Maltese Premier League 2001-02 alongside Lija Athletic, who finished runners-up. Tarxien and Zurrieq were relegated to the Maltese Second Division 2001-02. This was the second straight relegation for Zurrieq, having been relegated from the Premier League the previous season. Marsa suffered their only defeat at the hands of Zurrieq.

2000–01 Maltese First Division

The Maltese First Division 2000–01 (known as Rothmans First Division 2000–01 for sponsorship reasons) started on 9 September 2000 and finished on 29 April 2001. Gozo and Zurrieq were the relegated teams from the Maltese Premier League 1999-00. Marsaxlokk and Qormi were the promoted teams from the Maltese Second Division. Marsa finished as champions, by just one point, and were promoted to the Maltese Premier League 2001-02 alongside Lija Athletic, who finished runners-up. Tarxien and Zurrieq were relegated to the Maltese Second Division 2001-02. This was the second straight relegation for Zurrieq, having been relegated from the Premier League the previous season. Marsa suffered their only defeat at the hands of Zurrieq.