2000–01 National Division Two

The 2000–01 National Division Two was the fourteenth full season of rugby union within the third tier of the English league system currently known as National League 1. New teams to the division included West Hartlepool and Rugby Lions who were relegated from the 1999–2000 Allied Dunbar Premiership Two while promoted teams included Kendal and Esher who were promoted as champions of National Division Three North and National Division Three South respectively. The league system was 2 points for a win and 1 point for a draw.

2000–01 National Division Two

The 2000–01 National Division Two was the fourteenth full season of rugby union within the third tier of the English league system currently known as National League 1. New teams to the division included West Hartlepool and Rugby Lions who were relegated from the 1999–2000 Allied Dunbar Premiership Two while promoted teams included Kendal and Esher who were promoted as champions of National Division Three North and National Division Three South respectively. The league system was 2 points for a win and 1 point for a draw.