Acid guanidinium thiocyanate-phenol-chloroform extraction
Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This method may take longer than a column-based system such as the silica-based purification, but has higher purity and the advantage of high recovery of RNA: an RNA column is typically unsuitable for purification of short (<200 nucleotides) RNA species, such as siRNA, miRNA, gRNA and tRNA.
known for
1987 in scienceBCPBoom methodDNA microarrayGuanidinium thiocyanateGuanidinium thiocyanate-phenol-chloroform extractionGuanidium thiocyanate-phenol-chlorofrom extractionNicoletta SacchiNucleic acid quantitationPhenol–chloroform extractionRNA extractionSmall RNA sequencingSpin column-based nucleic acid purificationTosohTrizol
Link from a Wikipage to another Wikipage
known for
Acid guanidinium thiocyanate-phenol-chloroform extraction
Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This method may take longer than a column-based system such as the silica-based purification, but has higher purity and the advantage of high recovery of RNA: an RNA column is typically unsuitable for purification of short (<200 nucleotides) RNA species, such as siRNA, miRNA, gRNA and tRNA.
has abstract
Acid guanidinium thiocyanate-p ...... e; and by Tel-Test as STAT-60.
@en
Link from a Wikipage to an external page
Wikipage page ID
page length (characters) of wiki page
Wikipage revision ID
982,429,641
Link from a Wikipage to another Wikipage
wikiPageUsesTemplate
hypernym
type
comment
Acid guanidinium thiocyanate-p ...... s siRNA, miRNA, gRNA and tRNA.
@en
label
Acid guanidinium thiocyanate-phenol-chloroform extraction
@en