Barkers Creek is a tributary of the Guyandotte River, 8 miles (13 km) long, in southern West Virginia in the United States. Via the Guyandotte and Ohio rivers, it is part of the watershed of the Mississippi River, draining an area of 36.85 square miles (95.4 km2) in a rural area on the unglaciated portion of the Allegheny Plateau. According to 1992 data from the United States Geological Survey, approximately 94% of the Barkers Creek watershed was forested; approximately 2% was used for agriculture; and approximately 2% was used for commercial or mining purposes.