Sir Edward Chamberlain (or Chamberlayne) (1480 – 1543) of Shirburn Castle, Oxfordshire was an English soldier knight and Member of Parliament. He was born the eldest son of Richard Chamberlain of Coton, Northamptonshire and his wife Sybil Fowler and succeeded his father in 1497. His mother took out a lease on the Shirburn estate from her brother in 1505, which passed to Edward on her death in 1525. He served as High Sheriff of Berkshire and Oxfordshire for 1505-06 and 1517–18. He was made Keeper of Woodstock Park in 1508 and an Esquire of the Body in 1509.