Hanes–Woolf plot

In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against . It is based on the rearrangement of the Michaelis–Menten equation shown below: where is the Michaelis constant and is the limiting rate. because it is not a maximum in the mathematical sense. Starting from the Michaelis–Menten equation: we can take reciprocals of both sides of the equation to obtain the equation underlying the Lineweaver–Burk plot: · ·

Hanes–Woolf plot

In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against . It is based on the rearrangement of the Michaelis–Menten equation shown below: where is the Michaelis constant and is the limiting rate. because it is not a maximum in the mathematical sense. Starting from the Michaelis–Menten equation: we can take reciprocals of both sides of the equation to obtain the equation underlying the Lineweaver–Burk plot: · ·