Jean-Baptiste Lestiboudois (30 January 1715, Douai – 20 March 1804, Lille) was a French botanist and pharmacist. His son François Joseph Lestiboudois (1759-1815) and grandson Gaspard Thémistocle Lestiboudois (1797-1876) were also botanists. In 1770 he was appointed professor of botany by the magistrate of Lille, and from 1796 served as a professor of natural history at the École centrale du département du Nord à Lille.