The Jubilee Exhibition Railway was a railway spur in Adelaide, South Australia. It was built in 1887 from where the Adelaide station currently is to the Jubilee Exhibition Building which stood near Frome Road on what is now part of the University of Adelaide. The line used an existing tunnel under King William Road which had been built in 1854 for horses and cattle to cross the road without disrupting traffic. The tunnel is likely the origin of rumours about rail tunnels located under the city of Adelaide. Much of the land where the line once ran has been reused as parts of Adelaide University and the Adelaide Festival Centre. There were no stations built. No known evidence of the line remains today.