Lewes Presbyterian Church is a historic Presbyterian church building located at 100 Kings Highway in Lewes, Sussex County, Delaware. It was built in 1832, as a frame meeting house measuring 45 feet by 37 feet. In 1869, the church was renovated to add a number of a Gothic Revival style features and the addition of a chancel with Gothic windows. In 1886–1887, the tower and belfry were added and two massive Corinthian order columns were added to the interior. In 1931, the church acquired its Colonial Revival style front door. It was added to the National Register of Historic Places in 1977.