MRNA display
mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. The complex then binds to an immobilized target in a selection step (affinity chromatography). The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest.
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MRNA display
mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. The complex then binds to an immobilized target in a selection step (affinity chromatography). The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest.
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in vitro virus(インビトロウイルス、略称:IV ...... RNA display法と呼称し、その名称が一般化している。
@ja
mRNA display is a display tech ...... nd non-binders are washed off.
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in vitro virus(インビトロウイルス、略称:IV ...... RNA display法と呼称し、その名称が一般化している。
@ja
mRNA display is a display tech ...... for the molecule of interest.
@en
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In vitro virus
@ja
MRNA display
@en