Mexborough engine shed was an engine shed in Swinton, in South Yorkshire, England. It was built by the Great Central Railway and opened in 1875. The shed was built slightly to the west of the current Mexborough station on land between the River Don and the River Don Navigation. It had 15 dead end roads, and could handle about 150 steam locomotives, mainly for use on freight trains. The London and North Eastern Railway operated the shed from 1923. In 1948, on the formation of British Railways Eastern Region, Mexborough bore the shed code 36B, then 41F from 1958. It closed in February 1964.