Muhammad Ayub Khuhro (Urdu: محمد ایوب کھوڑو‎) (14 August 1901 – 1980) was a politician from Sindh, Pakistan, who became its first Chief Minister after independence of Pakistan and subsequently serving two additional terms in the 1950s. He later became the Defence Minister in the government of Prime Minister Feroz Khan Noon, before the imposition of martial law by General Ayub Khan. Hamida Khuhro, an academic, is his daughter and has written his biography.