The Pirie Street Brewery was a brewery situated on Pirie Street and Wyatt Streets, Adelaide, in the early days of the British colony of South Australia. It was succeeded on the same site after a few years by the Adelaide Brewery. Its original address was 50-62 Wyatt Street; today the buildings at 54–60 are heritage-listed in the South Australian Heritage Register, and there is a remaining building at 113 Pirie Street now occupied by the Hill Smith Gallery.