Ponerinae is a subfamily of ants in the Poneromorph subfamilies group, with about 1,600 species in 47 extant genera, including Dinoponera gigantea - one of the world's largest species of ant. Mated workers have replaced the queen as the functional egg-layers in several species of ponerine ants. In such queenless species, the reproductive status of workers can only be determined through ovarian dissections. Ponerinae is a subfamily of ants within the family of Formicidae. These ants typically nest in soil, forest litter, or rotting logs, and are predacious. They primarily prey on isopods. They mostly live in small colonies of up to 200 workers. They can be found mostly in tropical environments, but have been found in southeastern Canada and New York. Female workers have twelve segmented ant