St. James on-the-Lines (often written as St. James-on-the-Lines or St. James On-the-Lines) is a historic Anglican garrison church in Penetanguishene, Ontario. The church is still in use today serving as the Anglican parish church in Penetanguishene for services from Easter to Thanksgiving. The church is protected by a heritage conservation easement under the Ontario Heritage Act. There are two suggested origins for its full name. The first suggestion comes from its location on the lines of communication or road from the former military establishment at Penetanguishene to the south. The second possible meaning is from the old Victorian military usage of Lines for the rows of tents or buildings in a military camp. The church was constructed close to the original military establishment buildi