The Worthington Subdivision or Worthington Sub is a railway line operated by Union Pacific Railroad. It runs generally southwest-northeast and begins at a siding in St. James Township, Minnesota, where the Mankato Subdivision ends, and it continues to Sioux City, Iowa. The line has yard facilities in Worthington and Sioux City, as well as a small yard north of Worthington in Hersey and Lorain townships called Elk Creek. The line passes through many small towns and villages with grain elevators along its route, and it is the origin of numerous grain unit trains.