The 1965 Rugby League Lancashire Cup competition 1965 was the fifty-third occasion on which the Lancashire Cup completion had been held.Warrington won the trophy by beating Rochdale Hornets by the score of 16-5The match was played at Knowsley Road, Eccleston, St Helens, Merseyside, (historically in the county of Lancashire). The attendance was 21,360 and receipts were £3,800It would be almost another 20 years before the attendance would again exceed 20,000