Bioimage informatics is a subfield of bioinformatics and computational biology. It focuses on the use of computational techniques to analyze bioimages, especially cellular and molecular images, at large scale and high throughput. The goal is to obtain useful knowledge out of complicated and heterogeneous image and related metadata. There has been an increasing focus on developing novel image processing, computer vision, data mining, database and visualization techniques to extract, compare, search and manage the biological knowledge in these data-intensive problems.