The Chalmers Institute is a historic building in Holly Springs, Mississippi, USA. Built in 1837, it was home to the University of Holly Springs, the oldest university in Mississippi, from 1838 to 1839. It was home to a short-lived Methodist medical and law school from 1839 to 1843. It reopened as the Chalmers Institute, a Presbyterian boys' school, from 1850 to 1878, when a yellow fever epidemic closed down the school. It became home to the Holly Springs Normal Institute in 1879, but closed down a few years later. In the twentieth century, it became a private residence. It has been listed by the National Register of Historic Places for its historic significance since 1982.