Juanillo (? - May 1598) was a chief of the Native American Tolomato people in the Guale chiefdom, in what is now Georgia. In September 1597, Juanillo led the so-called Gualean Revolt, or Juanillo's Revolt, against the cultural oppression of the indigenous population in Florida by the Spanish authorities and the Franciscan missionaries. This was the first and longest-lasting Guale rebellion in La Florida, and ended with the execution of Juanillo by a group of Native American allies of the Spanish, led by Chief Asao.