The 1965 Florida Gators football team represented the University of Florida during the 1965 NCAA University Division football season. The season was Ray Graves's sixth year as the Florida Gators football team's head coach. The highlights of the season included an intersectional road victory over the Northwestern Wildcats of the Big Ten Conference, Southeastern Conference (SEC) wins over the LSU (14–7), Ole Miss Rebels (17–0), Georgia Bulldogs (14–10) and Tulane Green Wave (51–13), and a sound thumping of the in-state rival Florida State Seminoles (30–17). The Gators also lost close matches against the Mississippi State Bulldogs (13–18) and the Miami Hurricanes (13–16). Graves' 1965 Florida Gators finished 7–4 overall and 4–2 in the SEC, placing third in the eleven-team conference.