The 1965 Florida State Seminoles baseball team represented Florida State University in the 1965 NCAA University Division baseball season. The Seminoles played their home games at . The team was coached by Fred Hatfield in his second season at Florida State. The Seminoles reached the College World Series, their fourth appearance in Omaha, where they finished tied for fifth place after recording a second round win against Texas, and losses to eventual runner-up Ohio State and Saint Louis.