Amplified fragment length polymorphism
AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by , AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing on agarose gel electrophoresis , either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.
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Amplified fragment length polymorphism
AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by , AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing on agarose gel electrophoresis , either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.
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AFLP-PCR or just AFLP is a PCR ...... alisation of the band pattern.
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Amplification Fragment Length ...... ve of fluorescerende signalen.
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La AFLP-PCR o semplicemente AF ...... termini di espressione genica.
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Le polymorphisme de longueur d ...... n génétique et en transgénèse.
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Los polimorfismos en la longit ...... de biotecnología holandesa .
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Polimorfisme panjang fragmen t ...... aan bioteknologi asal Belanda.
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Ме́тод поліморфі́зму довжини́ ...... ворення бактеріального геному.
@uk
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AFLP-PCR or just AFLP is a PCR ...... illary sequencing instruments.
@en
Amplification Fragment Length ...... ntwikkeld en gepatenteerd door
@nl
La AFLP-PCR o semplicemente AF ...... termini di espressione genica.
@it
Le polymorphisme de longueur d ...... n génétique et en transgénèse.
@fr
Los polimorfismos en la longit ...... cual supone una ventaja consi
@es
Polimorfisme panjang fragmen t ...... aan bioteknologi asal Belanda.
@in
Ме́тод поліморфі́зму довжини́ ...... ворення бактеріального геному.
@uk
label
AFLP
@de
Amplification fragment length polymorphism
@nl
Amplified fragment length polymorphism
@en
Amplified fragment length polymorphism
@it
Polimorfisme panjang fragmen teramplifikasi
@in
Polimorfismos en la longitud de fragmentos amplificados
@es
Polymorphisme de longueur des fragments amplifiés
@fr
Поліморфізм довжини ампліфікованих фрагментів
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