Ann Gregory (July 25, 1912 – February 5, 1990) was an African-American amateur golfer. Black newspapers had called her "The Queen of Negro Women's Golf." As stated in Arthur Ashe's book, Hard Road to Glory, many observers called Gregory the best African-American female golfer of the 20th century. In 1971, Gregory was runner-up at the USGA Senior Women's Amateur, making her the first African-American to finish as runner-up in a USGA women's competition. In all, during her career, Gregory won nearly 300 tournaments.