The Cheddington to Aylesbury Line was an early railway branch line, opening in 1839. It was promoted by local people who formed the Aylesbury Railway to construct it, and it made a junction with the London and Birmingham Railway at Cheddington. That company worked the branch line, and when the L&BR merged with others in 1846 to form the London and North Western Railway, the line was in effect the Aylesbury branch of the LNWR. The passenger service was discontinued in 1953 and the line was closed completely in 1963.