Cowbane Prairie Natural Area Preserve is a 147-acre (59 ha) Natural Area Preserve located in Augusta County, Virginia, along the western slope of the Blue Ridge in the Shenandoah Valley. It preserves both mesic and wet prairie habitats, as well as a calcareous spring-fed marsh; these areas contain eleven regionally rare plants such as queen-of-the-prairie, blueflag iris, and marsh-speedwell. Although common in the Midwest, these plants occur only at a handful of sites in Virginia.