Ctenophthalmus pseudagyrtes is a species of fleas in the family Hystrichopsyllidae. It is widespread in North America, east of the Rocky Mountains, and is found mainly on small mammals. In Missouri, it has been recorded on the Virginia opossum (Didelphis virginiana), northern short-tailed shrew (Blarina brevicauda), eastern mole (Scalopus aquaticus), raccoon (Procyon lotor), eastern chipmunk (Tamias striatus), Florida woodrat (Neotoma floridana), prairie vole (Microtus ochrogaster), woodland vole (Microtus pinetorum), white-footed mouse (Peromyscus leucopus), including nests, marsh rice rat (Oryzomys palustris), hispid cotton rat (Sigmodon hispidus), house mouse (Mus musculus), and brown rat (Rattus norvegicus). Hosts recorded in Tennessee include the Virginia opossum, northern short-taile