Establishment Creek is a stream in Ste. Genevieve County in the U.S. state of Missouri. It is a tributary of the Mississippi River. The stream headwaters are at 37°52′02″N 90°17′53″W / 37.86722°N 90.29806°W between the communities of Sprott and Millers and the stream crosses under U.S. Route 55 and U.S. Route 61 southeast of Bloomsdale. The confluence with the Mississippi is at 38°01′40″N 90°06′45″W / 38.02778°N 90.11250°W. The stream is impounded as Lake Wanda Lee just south of Rocky Ridge.