Fluidic force microscopy (FluidFM) is a type of scanning probe microscopy, and is typically used on a standard inverted light microscope. The unique characteristic of FluidFM is that it introduces microscopic channels into AFM probes. Those channels can have an aperture of less than 300 nm, or 500 times thinner than a human hair. This nanometric features enables the handling of liquid volumes at the femtoliter (fL) scale as well as force controlled manipulations of sub-micron objects. Via the nanofluidic channels, substances can for example be inserted into single cells or cells can be isolated from a confluent layer.