G418
G418 (Geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II. G418 is an analog of neomycin sulfate, and has similar mechanism as neomycin. G418 is commonly used in laboratory research to select genetically engineered cells . In general for bacteria and algae concentrations of 5 μg/mL or less are used, for mammalian cells concentrations of approximately 400 μg/mL are used for selection and 200 μg/mL for maintenance. However, optimal concentration for resistant clones selection in mammalian cells
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G418
G418 (Geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II. G418 is an analog of neomycin sulfate, and has similar mechanism as neomycin. G418 is commonly used in laboratory research to select genetically engineered cells . In general for bacteria and algae concentrations of 5 μg/mL or less are used, for mammalian cells concentrations of approximately 400 μg/mL are used for selection and 200 μg/mL for maintenance. However, optimal concentration for resistant clones selection in mammalian cells
has abstract
G418 (Geneticin) is an aminogl ...... quire from 1 to up to 3 weeks.
@en
G418はゲンタマイシンに類似した構造のアミノグリコシド系抗 ...... ーモフィッシャー・サイエンティフィック) の登録商標である。
@ja
G418，即遺傳黴素（Geneticin），是一種氨基糖苷類 ...... 中，利用上述機制，可以使用G418篩選基因工程改造後的細胞。
@zh
Geneticin (G-418) ist eine ant ...... veränderten Zelllinien dient.
@de
La geneticina (G418) è un anti ...... sia procariote sia eucariote.
@it
La généticine (ou G418) est un ...... e classique est son disulfate.
@fr
alternative name
Geneticin
@en
O-2-Amino-2,7-didesoxy-(D)-gly ...... ranosyl- (1→6))-(D)-streptamin
@en
IUPAC name
(2R,3S,4R,5R,6S)-5-Amino-6-{[( ...... -1-hydroxyethyl]oxane-3,4-diol
@en
Wikipage page ID
10,992,237
page length (characters) of wiki page
Wikipage revision ID
1,017,482,814
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OtherNames
Geneticin
@en
O-2-Amino-2,7-didesoxy-(D)-glycero-α-(D)-gluco-heptopyranosyl--O--(D)-streptamin
@en
Verifiedfields
changed
@en
verifiedrevid
461,115,152
Watchedfields
changed
@en
wikiPageUsesTemplate
comment
G418 (Geneticin) is an aminogl ...... s selection in mammalian cells
@en
G418はゲンタマイシンに類似した構造のアミノグリコシド系抗 ...... ーモフィッシャー・サイエンティフィック) の登録商標である。
@ja
G418，即遺傳黴素（Geneticin），是一種氨基糖苷類 ...... 中，利用上述機制，可以使用G418篩選基因工程改造後的細胞。
@zh
Geneticin (G-418) ist eine ant ...... veränderten Zelllinien dient.
@de
La geneticina (G418) è un anti ...... sia procariote sia eucariote.
@it
La généticine (ou G418) est un ...... e classique est son disulfate.
@fr
label
G418
@en
G418
@ja
G418
@zh
Geneticin
@de
Geneticina
@it
Généticine
@fr