Jana Jonášová (born April 1943) is a Czech opera singer. One of the most important Czech coloratura sopranos of her generation, she has had an active international career at the world's major opera houses and concert stages for roughly four decades. As an opera singer she performed a varied repertoire from a variety of musical periods and in many languages. She drew particular acclaim for her portrayal of Mozart heroines and roles from the standard Czech repertory. In 1970 she joined the National Theatre in Prague where she was a member for more than three decades.