John Gillespie may refer to: * John H. Gillespie, evolutionary biologist * (1832–1897) state auditor and commissioner in Nebraska * Dizzy Gillespie (John Birks Gillespie, 1917–1993), jazz trumpeter * Jimmy Gillespie (John Imrie Gillespie, 1879–1943), Scottish rugby union player * John Ross Gillespie (born 1935), field hockey player and coach from New Zealand * John Gillespie (baseball) (1900–1954), Major League Baseball pitcher * John Gillespie (footballer, born 1872) (1872–?), Scottish footballer for Queen's Park FC and Scotland * John Gillespie (footballer, born 1873) (1873–?), Scottish footballer for Sunderland * John Gillespie (moderator) (1834–1912), Moderator of the General Assembly of the Church of Scotland in 1903/04 * John Hamilton Gillespie (1852–1923), Scottish-Americ