Route 16 is a 16.709-mile-long (26.891 km) highway in Lewis County, Missouri. The western terminus is at Route 6 east of Lewistown. The route travels eastward, through Monticello and over a few rivers. Route 16 then intersects U.S. Route 61 (US 61). The route ends at US 61 Business and Route B in Canton. The road was formerly part of Route 6, but it was rerouted in 1931. The old alignment was renumbered Route 96 that year. Ten years later, the route was renumbered to Route 16.