The Ontario Southland Railway Inc. is an independently held short line operator. The company was founded in 1992 to purchase 17 miles (27 km) of track between Tillsonburg and Ingersoll, Ontario from the Canadian Pacific Railway. In 2009, a second line was added, as 32 miles (51 km) of CPR track from Woodstock to St. Thomas, Ontario were integrated into the system. In 2015, Ontario Southland began leasing the ex-Canadian National Cayuga Subdivision between St. Thomas and Delhi, Ontario, which had been abandoned by its former shortline operator the St. Thomas and Eastern Railway in 2013. The OSR also operated the Guelph Junction Railway until August 28th, 2020, when they ran their last train on the line before transferring operations to the Goderich and Exeter Railway.