Paired-end tag
Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites). Paired-end tags (PET) exist in PET libraries with the intervening DNA absent, that is, a PET "represents" a larger fragment of genomic or cDNA by consisting of a short 5' linker sequence, a short 5' sequence tag, a short 3' sequence tag, and a short 3' linker sequence. It was shown conceptually that 13 base pairs are sufficient to map tags uniquely.
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Cap analysis gene expressionChIA-PETChIP sequencingChromatin immunoprecipitationContigEpigenomicsMate-pairMate pairMate pairsMetagenomicsOptical mappingPETPaired-End TagsPaired-end TagsPaired-end tagsPaired end tagsPolony sequencingSerial analysis of gene expressionShotgun sequencingThird-generation sequencing
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Paired-end tag
Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites). Paired-end tags (PET) exist in PET libraries with the intervening DNA absent, that is, a PET "represents" a larger fragment of genomic or cDNA by consisting of a short 5' linker sequence, a short 5' sequence tag, a short 3' sequence tag, and a short 3' linker sequence. It was shown conceptually that 13 base pairs are sufficient to map tags uniquely.
has abstract
Paired-end tags (PET) (sometim ...... y one end of the DNA fragment.
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Секвенирование спаренных концо ...... /кДНК соединены друг с другом.
@ru
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comment
Paired-end tags (PET) (sometim ...... ficient to map tags uniquely.
@en
Секвенирование спаренных концо ...... /кДНК соединены друг с другом.
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label
Paired-end tag
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Секвенирование спаренных концов
@ru