Shire Brook is a small stream in the south eastern part of the City of Sheffield in South Yorkshire, England. It rises in the suburb of Gleadless Townend and flows in a general easterly direction for 4 miles (6.5 km) to its confluence with the River Rother between Beighton and Woodhouse Mill. In the past the brook has been both the border of Yorkshire and Derbyshire and between the sees of Canterbury and York. The course of the stream has been influenced by human intervention in the 20th century with the brook being diverted underground and flowing through culverts on three occasions as it traverses locations which were formerly landfill sites and extensive railway sidings.